Application
WB analysis of AKT1, AKT2, AKT3 and DSBA recombinant antigen fragments using DSBA antibody (left) or pan-AKT antibody at a dilution of 1:1,000.Application
WB analysis of 3T3, 293T, PC12 and K562 lysates using pan-AKT antibody at a dilution of 1:1,000.Application
IP analysis of HeLa lysate using aontrol IgG and pan-AKT antibody. The precipitates were delected by the same pan-AKT antibody.| Product Name | AKT antibody [3B11-G8-B1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [3B11-G8-B1] to total pan-AKTAkt1 is involved in cellular survival pathways,by inhibiting apoptotic processes.Akt1 is also able to induce protein synthesis pathways,and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth.Since it can block apoptosis,and thereby promote cell survival,Akt1 has been implicated as a major factor in many types of cancer.Akt(now also called Akt1)was originally identified as the oncogene in the transforming retrovirus,AKT8. Akt2 is an important signaling molecule in the Insulin signaling pathway.It is required to induce glucose transport.In a mouse which is null for Akt1 but normal for Akt2,glucose homeostasis is unperturbed,but the animals are smaller,consistent with a role for Akt1 in growth.In contrast,mice which do not have Akt2,but have normal Akt1,have mild growth deficiency and display a diabetic phenotype(insulin resistance),again consistent with the idea that Akt2 is more specific for the insulin receptor signaling pathway. The role of Akt3 is less clear,though it appears to be predominantly expressed in the brain.It has been reported that mice lacking Akt3 have small brains. |
| Immunogen | Purified recombinant human AKT protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3B11-G8-B1 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | v-akt murine thymoma viral oncogene homolog 1 , AKT1 , RAC , CWS6 , PRKBA , RAC-ALPHA , PKB , PKB-ALPHA , AKT |
|---|---|
| Target | AKT |
| Molecular Weight(MW) | 60kDa (note) |
| Cellular Localization | Cytoplasm. Nucleus. Cell membrane. |
Application
WB analysis of AKT1, AKT2, AKT3 and DSBA recombinant antigen fragments using DSBA antibody (left) or pan-AKT antibody at a dilution of 1:1,000.
Application
WB analysis of 3T3, 293T, PC12 and K562 lysates using pan-AKT antibody at a dilution of 1:1,000.
Application
IP analysis of HeLa lysate using aontrol IgG and pan-AKT antibody. The precipitates were delected by the same pan-AKT antibody.| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol. |
