Application
WB analysis of indicated cell lysates using AKT1 antibody at a dilution of 1:1000.| Product Name | AKT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to AKT1The protein kinase B (PKB, also called Akt) plays a key role in insulin signalling, cellular survival, and transformation. Akt1 is activated by phosphorylation on residues threonine 308 and Serine 473. Thr308, in the catalytic domain, is phosphorylated by the protein kinase PDK1, and Ser473, in the COOH-terminal regulatory domain, is phosphorylated by putative serine 473 kinase. To date, a couple of proteins have been shown to physically interact with PKB. |
| Immunogen | Synthetic peptide (Human) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| For ELISA: Use at a concentration of For IP: Use at a concentration of 3.0-5.0 μg/extract. For WB: Use at a concentration of 0.5-2.0 μg/ml. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | RAC, 207, MGC99656, 164730, AKT1, P31749, PRKBA, RAC-ALPHA, RACALPHA, PKB, RAC ALPHA |
|---|---|
| Target | AKT1 |
Application
WB analysis of indicated cell lysates using AKT1 antibody at a dilution of 1:1000.| Application Notes | For ELISA: Use at a concentration of For IP: Use at a concentration of 3.0-5.0 μg/extract. For WB: Use at a concentration of 0.5-2.0 μg/ml. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH .4, containing 0.05% sodium azide |
