Application
Anti-AKT3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with AKT3Application
HEK293T cells transfected with either AKT3 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AKT3 antibody , and then analyzed by flow cytometry.Application
AKT3 antibody ( 9B2 ) at 1:500 dilution + BALB/C 3T3 cell lysateApplication
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.Application
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-AKT3 mouse monoclonal antibody.| Product Name | AKT3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [9B2] to Akt3IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. |
| Immunogen | Recombinant fragment expressed in E.coli corresponding to amino acids 2-479 of human AKT3 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | 9B2 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRKBG DKFZP434N0250 AKT3 RACgamma PKBG STK2 RAC-gamma RAC-PK-gamma RACPKgamma STK-2 STK 2 RAC gamma RAC PK gamma |
|---|---|
| Target | Akt3 |
| Tissue Specificity | is specific for amino acids 2-479 of human AKT3 |
| Cellular Localization | Membrane-associated after cell stimulation leading to its translocation. |
Application
Anti-AKT3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with AKT3
Application
HEK293T cells transfected with either AKT3 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AKT3 antibody , and then analyzed by flow cytometry.
Application
AKT3 antibody ( 9B2 ) at 1:500 dilution + BALB/C 3T3 cell lysate
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.
Application
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-AKT3 mouse monoclonal antibody.| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
