Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT3 antibody [GT169] diluted at 1:1000.Application
AKT3 antibody [GT169] detects AKT3 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. AKT3 antibody [GT169] diluted at 1:200.Application
AKT3 antibody [GT169] detects AKT3 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. AKT3 antibody [GT169] diluted at 1:200.| Product Name | AKT3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT169] to AKT3 (v-akt murine thymoma viral oncogene homolog 3)The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human AKT3. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT169 |
| Host Species | Mouse |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rhesus Monkey, Chimpanzee, BovinePig (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AKT3 antibody MPPH antibody PKB GAMMA antibody PKBG antibody PRKBG antibody RAC gamma antibody RAC-PK gamma antibody STK2 antibody v-akt murine thymoma viral oncogene homolog 3 antibody |
|---|---|
| Target | AKT3 |
| Molecular Weight(MW) | 56 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Membrane |
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT3 antibody [GT169] diluted at 1:1000.
Application
AKT3 antibody [GT169] detects AKT3 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. AKT3 antibody [GT169] diluted at 1:200.
Application
AKT3 antibody [GT169] detects AKT3 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. AKT3 antibody [GT169] diluted at 1:200.| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
