Application
Sample (50 ug of whole cell lysate) A: Mouse liver 5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
alanyl-tRNA synthetase antibody [N3C2], Internal detects AARS protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. alanyl-tRNA synthetase antibody [N3C2], Internal dilution: 1:500.| Product Name | alanyl-tRNA synthetase antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to alanyl-tRNA synthetase (alanyl-tRNA synthetase)The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alanyl-tRNA synthetase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, BovineRat (92%), Xenopus laevis (84%), Chicken (86%), Bovine (92%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CMT2N antibody AARS antibody alanine tRNA ligase 1 cytoplasmic antibody alanine--tRNA ligase antibody renal carcinoma antigen NY-REN-42 antibody alaRS antibody alanyl-tRNA synthetase cytoplasmic antibody alanyl-tRNA synthetase antibody |
|---|---|
| Molecular Weight(MW) | 107 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (50 ug of whole cell lysate) A: Mouse liver 5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
alanyl-tRNA synthetase antibody [N3C2], Internal detects AARS protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. alanyl-tRNA synthetase antibody [N3C2], Internal dilution: 1:500.| Positive Control | A431 , H1299 , HeLa , HepG2 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
