Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1L1 monoclonal antibody.Application
HEK293T cells transfected with either RC213720 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ALDH1L1 antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH1L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH1L1.Application
Anti-ALDH1L1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with ALDH1L1Application
Flow cytometric Analysis of Hela cells, using anti-ALDH1L1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).| Product Name | ALDH1L1 antibody [5G8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5G8] to ALDH1L1The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning. [provided by RefSeq] |
| Immunogen | Full length human recombinant protein of human ALDH1L1 (NP_036322) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 5G8 |
| Host Species | Mouse |
| Tested Applications | FACS,ICC/IF,WB |
| ICC/IF 1:100 | |
| Species Reactivity | Human,Dog |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FTHFD, DKFZp781N0997, 10fTHF, ALDH1L1 |
|---|---|
| Target | ALDH1L1 |
| Tissue Specificity | is specific for human ALDH1L1 (NP_036322). |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1L1 monoclonal antibody.
Application
HEK293T cells transfected with either RC213720 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ALDH1L1 antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH1L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH1L1.
Application
Anti-ALDH1L1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with ALDH1L1
Application
Flow cytometric Analysis of Hela cells, using anti-ALDH1L1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
