Application
ICC/IF analysis of A549 cells using DAPI (blue) and Aldolase A antibody [AT3F9] at a dilution of 1:200 (green).Application
WB analysis of (1) recombinant human Aldolase A, (2) HeLa and (3) HepG2 lysates (40ug of lysates) using Aldolase A antibody [AT3F9] at a dilution of 1:1000.Application
FACS analysis of A549 cells using an isotype control (black) or Aldolase A antibody [AT3F9] at 2-5ug for 1,000,000 cells (red).| Product Name | Aldolase A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [AT3F9] to Aldolase AThe protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Aug 2011] |
| Immunogen | Recombinant human ALDOA (1-364aa) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | AT3F9 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| WB: 1:1000. ICC/IF: 1:200. FACS: 1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ALDOA GSD12 ALDA aldolase A fructose-bisphosphate |
|---|---|
| Target | Aldolase A |
| Light Chain Type | kappa |
Application
ICC/IF analysis of A549 cells using DAPI (blue) and Aldolase A antibody [AT3F9] at a dilution of 1:200 (green).
Application
WB analysis of (1) recombinant human Aldolase A, (2) HeLa and (3) HepG2 lysates (40ug of lysates) using Aldolase A antibody [AT3F9] at a dilution of 1:1000.
Application
FACS analysis of A549 cells using an isotype control (black) or Aldolase A antibody [AT3F9] at 2-5ug for 1,000,000 cells (red).| Application Notes | WB: 1:1000. ICC/IF: 1:200. FACS: 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -27C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol |
