Application
IF - alpha 1 Spectrin antibody [17C7]| Product Name | alpha 1 Spectrin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [17C7] to alpha 1 SpectrinSpectrin is the predominant component of the membrane skeleton of the red blood cell and is essential in determining shape and deformability of the membrane. It consists of 2 nonidentical subunits, alpha (MW 240,000) and beta (MW 225,000). Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network. |
| Immunogen | Recombinant alpha I spectrin (erythroid spectrin) SH3 domain. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 17C7 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-FWB |
| IF: Use at a dilution of 1/100 - 1/500. IHC-Fr: Use at a dilution of 1/50 - 1/100. WB: Use at a dilution of 1/100 - 1/1000. Predicted molecular weight: 280 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EL2 SPTA1 alpha 1 Spectrin 6708 alpha1 Spectrin 182860 P02549 HPP HS3 SPH3 SPTA |
|---|---|
| Target | alpha 1 Spectrin |
| Molecular Weight(MW) | 280 kDa(Predicted) |
| Tissue Specificity | is developed against the alpha I spectrin SH3 domain. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In NIH 3T3 cells, reacts with a 200 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes. |
| Cellular Localization | Anchored to the cytoplasmic face of the plasma membrane. |
Application
IF - alpha 1 Spectrin antibody [17C7]| Positive Control | Erythroid spectrin in red blood cells and cerebellum |
|---|---|
| Application Notes | IF: Use at a dilution of 1/100 - 1/500. IHC-Fr: Use at a dilution of 1/50 - 1/100. WB: Use at a dilution of 1/100 - 1/1000. Predicted molecular weight: 280 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS, Ammonium sulphate |
