Application
Mouse eye extracts and recombinant proteins (Crystallin-alpha A and B) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Crystallin alpha A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody con| Product Name | alpha A Crystallin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [c9F2] to alpha A Crystallinalpha-crystallins, a major protein of the ocular lens, are thought to play a role in maintaining lens transparency, which are composed of two gene products alpha-A and alpha-B, for acidic and basic, respectively. alpha-crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP). They act as molecular chaperones and hold unfolded or misfolded proteins in large, water-soluble low molecular weight aggregates. These heterogeneous aggregates consist of 30-40 subunits of the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of alpha-crystallins are an autokinase activity and the participation in the intracellular architecture. |
| Immunogen | Recombinant human alpha A Crystallin (1-173aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | c9F2 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western blot analysis: 1:500 ~ 1:2000. Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CRYAA Alphacrystallin A Crystallin Alpha 1 HSPB4 Alpha-crystallin A chain CRYA 1 Acry1 Heat shock protein beta-4 HSPB 4 CRYA1 Heat shock protein beta4 Crystallin alpha A Alpha crystallin A chain Crystallin Alpha1 Acry-1 Alphacrystallin A cha |
|---|---|
| Target | alpha A Crystallin |
| Cellular Localization | Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. |
| Light Chain Type | kappa |
Application
Mouse eye extracts and recombinant proteins (Crystallin-alpha A and B) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Crystallin alpha A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody con| Application Notes | Western blot analysis: 1:500 ~ 1:2000. Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
