Application
Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-ACTN1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ACTN1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ACTN1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-ACTN1 mouse monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACTN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACTN1.Application
Anti-ACTN1 mouse monoclonal antibody immunofluorescent staining of Hela cells transiently transfected with ACTN1Application
Immunofluorescent staining of HeLa cells using anti-ACTN1 mouse monoclonal antibody .Application
ACTN1 antibody (7A4) at 1:2000 + recombinant human ACTN1Application
Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-ACTN1 mouse monoclonal antibody.| Product Name | alpha Actinin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [7A4] to alpha-actininF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 650-893 of human alpha-actinin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 7A4 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ40884 ACTN1 FLJ54432 alpha Actinin alphaActinin |
|---|---|
| Target | alpha-actinin |
| Tissue Specificity | is specific for amino acids 650-893 of human alpha-actinin |
| Cellular Localization | Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. |
Application
Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-ACTN1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ACTN1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-ACTN1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-ACTN1 mouse monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACTN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACTN1.
Application
Anti-ACTN1 mouse monoclonal antibody immunofluorescent staining of Hela cells transiently transfected with ACTN1
Application
Immunofluorescent staining of HeLa cells using anti-ACTN1 mouse monoclonal antibody .
Application
ACTN1 antibody (7A4) at 1:2000 + recombinant human ACTN1
Application
Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-ACTN1 mouse monoclonal antibody.| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
