Application
HEK293T cells transfected with either CRYAB overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody , and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-CRYABmouse monoclonal antibody. ( Dilution 1:50)Application
Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CRYABApplication
Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody .| Product Name | alpha B Crystallin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4A6] to CRYABMay contribute to the transparency and refractive index of the lens. |
| Immunogen | Protein expressed in 293T cell transfected with human CRYAB expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4A6 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| Flow 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CRYAB CRYA2 HSPB5 CTPP2 |
|---|---|
| Target | CRYAB |
| Tissue Specificity | is specific for human CRYAB. |
| Cellular Localization | Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. |
Application
HEK293T cells transfected with either CRYAB overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody , and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB.
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-CRYABmouse monoclonal antibody. ( Dilution 1:50)
Application
Anti-CRYAB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with CRYAB
Application
Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody .| Application Notes | Flow 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
