Application
Flow cytometric Analysis of Jurkat cells, using anti-AFP antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-AFP mouse monoclonal antibody.Application
Anti-AFP mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with AFPApplication
HEK293T cells transfected with either RC206622 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AFP antibody, and then analyzed by flow cytometry.| Product Name | Alpha fetoprotein / AFP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2A9] to AFPBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. |
| Immunogen | Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 2A9 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanDogMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FETA AFP HPAFP |
|---|---|
| Target | AFP |
| Tissue Specificity | is specific for human AFP (NP_001125). |
Application
Flow cytometric Analysis of Jurkat cells, using anti-AFP antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-AFP mouse monoclonal antibody.
Application
Anti-AFP mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with AFP
Application
HEK293T cells transfected with either RC206622 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AFP antibody, and then analyzed by flow cytometry.| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
