Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody.Application
HEK293T cells transfected with either RC206622 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AFP antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.| Product Name | Alpha fetoprotein / AFP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3B5] to AFPBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. |
| Immunogen | Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3B5 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| ICC/IF 1:50~100,: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FETA AFP HPAFP |
|---|---|
| Target | AFP |
| Tissue Specificity | is specific for human AFP (NP_001125). |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody.
Application
HEK293T cells transfected with either RC206622 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-AFP antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.| Application Notes | ICC/IF 1:50~100,: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
