Mouse monoclonal antibody [6E6] to AFPThis gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
Immunogen
The immunogen corresponding to human AFP expressed in E. Coli.
Key Feature
Clonality
Monoclonal
Isotype
IgG2
Clone Number
6E6
Host Species
Mouse
Tested Applications
ELISAWB
ELISA: 1/10000.:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Target Information
Gene Synonyms
FETA HPAFP
Target
AFP
Molecular Weight(MW)
69 kDa (note)
Application
Application
Western blot analysis using anti-AFP mAb against HepG2 (1) and SMMC-7721 (2) cell lysate.
Application Notes
ELISA: 1/10000.:
Additional Information
Form
Liquid
Storage Instructions
Store at 4℃, for long term storage, store at -20℃.
Storage Buffer
Antibody are purified by protein G affinity chromatography. Liquid in PBS containing 50% glycerol and 0.03% sodium azide.