| Product Name | Alpha fetoprotein / AFP antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [C3] to alpha 1 Fetoprotein predilutedAlpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries. |
| Immunogen | BALB/C mice were injected with human alpha-fetoprotein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | C3 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be alldetermined by the user.: |
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| Species Reactivity | HumanDogPig |
| Concentration | 1mg/ml |
| Gene Synonyms | HPAFP alpha1 Fetoprotein 174 FETA 104150 AFP alpha 1 Fetoprotein P02771 |
|---|---|
| Target | alpha 1 Fetoprotein |
| Tissue Specificity | Alpha-Fetoprotein (AFP) is a single chain glycoprotein of 70 kDa that is normally expressed in the fetal liver, gastrointestinal tract and yolk sac. This antibody recognizes an epitope of alpha - Fetoprotein present in human and several other species. |
| Cellular Localization | Secreted |
| Application Notes | This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be alldetermined by the user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH7.2 containing 0.05% sodium azide |
