Application
Application| Product Name | alpha Sarcomeric Actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5C5] to alpha Sarcomeric ActinMonoclonal anti-alpha-sarcomeric Actin has been used as a marker for rhabdomyosarcoma. This antibody is specific to alpha-skeletal and alpha-cardiac muscle actins. It cross reacts with human, sheep, bovine, rabbit, guinea pig, rat, frog, snake and carp. It does not reacts with smooth muscle tissue. |
| Immunogen | BALB/C mice were injected with purified rabbit striated muscle actin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | 5C5 |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Target | alpha Sarcomeric Actin |
|---|---|
| Cellular Localization | Cytoplasmic |
Application
Application| Positive Control | Skeletal muscle |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | This antibody is supplied as diluted ascites containing |
