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Immunohistochemistry (Paraffin/Frozen). Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:25 to 1:50 with AutoProbe III (GTX28620) . The optimal conditions should be determined by the individual laboratory. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies.:
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