Application
Chinese hamster embryonic fibroblasts in anaphase of mitosis were stained using Monoclonal Anti-α-Tubulin, clone no. DM1A with Anti-Mouse-Alexa 488 (green) and DAPI (blue).Application
Confocal image of the mouth of the Cryptocaryon irritans, a parasite of marine fishes, (theront stage) stained with Monoclonal Anti-α-Tubulin, clone no. DM 1A and Anti-Mouse IgG-lissamine-rhodamine conjugate. Note the large bundles of microtubules which form a well-developed cytophyrigeal basket.Application
IHC-P analysis of human heart sections using alpha Tubulin antibody [DM1A] at a dilution of 1:500.Application
WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [DM1A] at a dilution of 1:500.Application
Whole cell extract of chicken gizzard was separated on SDS-PAGE and blotted with Monoclonal Anti-α-Tubulin . The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lane A: Antibody dilution 1:1,000 Lane B: Negative control (only secondary antibody)Application
Human skin fibroblasts stained with Monoclonal Anti-α-Tubulin, clone No. DM1A .| Product Name | alpha Tubulin antibody [DM1A] (ascites) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [DM1A] to alpha TubulinTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha-tubulin and beta-tubulin; both subunits have a molecular weight of approximately 50 kDa and share considerable homology. The most widely studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.GT211302 may be used to study the intracellular organization and distribution of tubulin along the static and dynamic aspects of the cytoskeleton. The product can be used for the immunocytochemical localization of the alpha chain of tubulin by means of indirect immunofluorescent labeling of cultured cells, formalin fixed or frozen tissues or for specific identification of the alpha-tubulin band in immunoblotting or immunoprecipitation techniques. |
| Immunogen | Purified chick brain tubulin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | DM1A |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PIPWBIB |
| IF: Use at a dilution of 1/500, this is determined by indirect immunofluorescent labeling of cultured chicken fibroblasts. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseAmphibiansFishFungiHamsterBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TUBA1, P68366, alpha Tubulin, 191110, H2ALPHA, H2-ALPHA, 7277, FLJ30169, TUBA1B, TUBA2, TUBA3D, TUBA8, H2 ALPHA |
|---|---|
| Target | alpha Tubulin |
| Cellular Localization | Major constituent of microtubules. |
Application
Chinese hamster embryonic fibroblasts in anaphase of mitosis were stained using Monoclonal Anti-α-Tubulin, clone no. DM1A with Anti-Mouse-Alexa 488 (green) and DAPI (blue).
Application
Confocal image of the mouth of the Cryptocaryon irritans, a parasite of marine fishes, (theront stage) stained with Monoclonal Anti-α-Tubulin, clone no. DM 1A and Anti-Mouse IgG-lissamine-rhodamine conjugate. Note the large bundles of microtubules which form a well-developed cytophyrigeal basket.
Application
IHC-P analysis of human heart sections using alpha Tubulin antibody [DM1A] at a dilution of 1:500.
Application
WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [DM1A] at a dilution of 1:500.
Application
Whole cell extract of chicken gizzard was separated on SDS-PAGE and blotted with Monoclonal Anti-α-Tubulin . The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lane A: Antibody dilution 1:1,000 Lane B: Negative control (only secondary antibody)
Application
Human skin fibroblasts stained with Monoclonal Anti-α-Tubulin, clone No. DM1A .| Positive Control | Chicken fibroblasts |
|---|---|
| Application Notes | IF: Use at a dilution of 1/500, this is determined by indirect immunofluorescent labeling of cultured chicken fibroblasts. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 50 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Raw Ascites containing 15mM sodium azide |
