Application
Western blot analysis using anti-AMACR mAb against Jurkat (1) and LNCaP (2) cell lysate.Application
Confocal immunofluorescence analysis of LNCaP cells using AMACR mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin Blue: DRAQ5 fluorescent DNA dyeApplication
Immunohistochemical analysis of paraffin-embedded human brain cerebellum using anti-AMACR mAb.Application
Immunohistochemical analysis of paraffin-embedded human normal prostate tissues (left) and prostate adenocarcinoma tissues (right), showing cytoplasmic localization using anti-AMACR mAb with DAB staining.| Product Name | AMACR antibody [2A10F3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2A10F3] to AMACRThis gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011] |
| Immunogen | The immunogen corresponding to human AMACR expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 2A10F3 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-PWB |
| Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. ICC: 1/200 - 1/1000. Not yet tested in other applications. Determining optimal working dilutions by titration test. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Synonyms | CBAS4 , RACE , AMACRD , RM |
|---|---|
| Target | AMACR |
| Molecular Weight(MW) | 42 kDa (note) |
Application
Western blot analysis using anti-AMACR mAb against Jurkat (1) and LNCaP (2) cell lysate.
Application
Confocal immunofluorescence analysis of LNCaP cells using AMACR mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin Blue: DRAQ5 fluorescent DNA dye
Application
Immunohistochemical analysis of paraffin-embedded human brain cerebellum using anti-AMACR mAb.
Application
Immunohistochemical analysis of paraffin-embedded human normal prostate tissues (left) and prostate adenocarcinoma tissues (right), showing cytoplasmic localization using anti-AMACR mAb with DAB staining.| Application Notes | Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. ICC: 1/200 - 1/1000. Not yet tested in other applications. Determining optimal working dilutions by titration test. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
