Application
WB analysis of human brain tissue lysate using AMPK alpha 1/2 antibody [34.2].| Product Name | AMPK alpha 1/2 antibody [34.2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [34.2] to AMPK alpha 1/2The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008] |
| Immunogen | 29 amino acid peptide within the N-terminal catalytic domain of Drosophila AMPK alpha. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 34.2 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-FIPWB |
| Western Blotting: 1/100 - 1/1000 Optimal dilution / concentration should be determined by the end user. | |
| Species Reactivity | HumanMouseDrosophilaRatVertebrates |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AMPKa2 , PRKAA2 , AMPK , protein kinase, AMP-activated, alpha 2 catalytic subunit , PRKAA , AMPK2 |
|---|---|
| Target | AMPK alpha 1/2 |
| Tissue Specificity | This antibody recognizes both the alpha 1 and the alpha 2 isoforms of AMP-activated protein kinase (AMPK). |
Application
WB analysis of human brain tissue lysate using AMPK alpha 1/2 antibody [34.2].| Application Notes | Western Blotting: 1/100 - 1/1000 Optimal dilution / concentration should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide (NaN3) |
