Application
WB analysis of human brain tumour tissue lysate using AMPK alpha 1/2 antibody [84.1].| Product Name | AMPK alpha 1/2 antibody [84.1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [84.1] to AMPK alpha 1/2The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| Immunogen | 29 amino acid peptide within the N-terminal catalytic domain of Drosophila AMPK alpha. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 84.1 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-FIPWB |
| Western Blotting: 1/100 - 1/1000 Optimal dilution / concentration should be determined by the end user. | |
| Species Reactivity | Drosophila |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AMPK , PRKAA1 , AMPKa1 , protein kinase, AMP-activated, alpha 1 catalytic subunit |
|---|---|
| Target | AMPK alpha 1/2 |
| Tissue Specificity | This antibody recognizes both the alpha 1 and the alpha 2 isoforms of AMP-activated protein kinase (AMPK). |
Application
WB analysis of human brain tumour tissue lysate using AMPK alpha 1/2 antibody [84.1].| Application Notes | Western Blotting: 1/100 - 1/1000 Optimal dilution / concentration should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide (NaN3) |
