Application
Immunofluorescence of APC5 in rat kidney tissue with APC5 antibody at 20 μg/mL.Application
Western blot analysis of APC5 in human kidney tissue lysate with APC5 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of APC5 in rat kidney tissue with APC5 antibody at 5 μg/mL.| Product Name | ANAPC5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ANAPC5This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008] |
| Immunogen | APC5 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 1 - 2 ug/ml. IHC: 5 ug/ml. IF: 20 ug/ml | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | anaphase promoting complex subunit 5 , APC5 |
|---|---|
| Target | ANAPC5 |
Application
Immunofluorescence of APC5 in rat kidney tissue with APC5 antibody at 20 μg/mL.
Application
Western blot analysis of APC5 in human kidney tissue lysate with APC5 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of APC5 in rat kidney tissue with APC5 antibody at 5 μg/mL.| Application Notes | WB: 1 - 2 ug/ml. IHC: 5 ug/ml. IF: 20 ug/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stored At 4ºc For Three Months And -20ºc, Stable For Up To One Year. As With All Antibodies Care Should Be Taken To Avoid Repeated Freeze Thaw Cycles. Antibodies Should Not Be Exposed To Prolonged High Temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
