Application
Immunohistochemical analysis of paraffin-embedded human prostate tissues using Androgen receptor mouse mAbApplication
Western blot analysis using Androgen receptor mAb against human recombinant Androgen receptor.Application
Western blot analysis using Androgen receptor mAb against Androgen receptor (aa221-321)-hIgGFc transfected HEK293 cell lysate.Application
Western blot analysis using Androgen receptor mAb against K562 (1), Jurkat (2) and LNCaP (3) cell lysate.Application
Immunohistochemical analysis of paraffin-embedded Human testis (left) and prostate cancer (right) tissues using Androgen Receptor mouse mAb with DAB staining| Product Name | Androgen Receptor antibody [2H8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2H8] to Androgen receptorThe androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 221 and 321 of human Androgen Receptor. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2H8 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. Not yet tested in other applications. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AIS , AR , SBMA , HUMARA , DHTR , HYSP1 , KD , NR3C4 , SMAX1 , TFM |
|---|---|
| Target | Androgen receptor |
| Molecular Weight(MW) | 110 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded human prostate tissues using Androgen receptor mouse mAb
Application
Western blot analysis using Androgen receptor mAb against human recombinant Androgen receptor.
Application
Western blot analysis using Androgen receptor mAb against Androgen receptor (aa221-321)-hIgGFc transfected HEK293 cell lysate.
Application
Western blot analysis using Androgen receptor mAb against K562 (1), Jurkat (2) and LNCaP (3) cell lysate.
Application
Immunohistochemical analysis of paraffin-embedded Human testis (left) and prostate cancer (right) tissues using Androgen Receptor mouse mAb with DAB staining| Application Notes | Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. Not yet tested in other applications. Determining optimal working dilutions by titration test. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
