Application
WB analysis of (1) BSA, (2) recombinant protein AR and (3) protein marker using Androgen Receptor antibody [3C6-F12-F11] at a concentration of 0.5 μg/ml.| Product Name | Androgen Receptor antibody [3C6-F12-F11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3C6-F12-F11] to Androgen ReceptorThe androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 2 and 556 of human Androgen Receptor. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3C6-F12-F11 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Recommended Starting Dilutions: For ELISA: Use at a concentration of 12.5 ng/ml. For WB: Use at a dilution of 1:2,000. Optimal working dilutions should be determined experimentally be the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AIS , DHTR , SMAX1 , NR3C4 , HYSP1 , TFM , KD , HUMARA , SBMA |
|---|---|
| Target | Androgen Receptor |
| Tissue Specificity | The Mouse anti-human AR antibody specifically detects targets derived from human recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity. |
Application
WB analysis of (1) BSA, (2) recombinant protein AR and (3) protein marker using Androgen Receptor antibody [3C6-F12-F11] at a concentration of 0.5 μg/ml.| Application Notes | Recommended Starting Dilutions: For ELISA: Use at a concentration of 12.5 ng/ml. For WB: Use at a dilution of 1:2,000. Optimal working dilutions should be determined experimentally be the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C if intended for use within one month, otherwise, store at -20 °C to -80 °C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20 °C to -80°C. After reconstitution, it can be aliquoted and store |
| Storage Buffer | PBS |
