Application
Structure of the recombinant antibody anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a mouse IgG2b Fc region.Application
Binding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by the antibody anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free). Methods: Tie-2 (h):Fc was coated on an ELISA plate at 1µg/ml. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free) or an unrelated mAb (recombinant) (Control) were added (starting at 40µg/ml with a twofold serial dilution) together with 20ng/µl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).| Product Name | Angiopoietin 2 antibody [Angy-2-1] (Azide free) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [Angy-2-1] to Angiopoietin 2The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant human angiopoietin-2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | Angy-2-1 |
| Host Species | Mouse |
| Tested Applications | ELISAFunctional Assay |
| Functional: Inhibits the binding of mouse angiopoietin-2 to mouse Tie-2. ND50 = 50-60ng/ml (for 10ng/ml of mouse Angiopoietin-2) inhibits the binding of human angiopoietin-2 to human Tie-2. ND50 = 8-12ng/ml (for 10ng/ml of human Angiopoietin-2) ND50: 50% neutralizing dose of antibody for a given concentration of ligand (here Angiopoietin-2). | |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | angiopoietin 2 , ANGPT2 , ANG2 , AGPT2 |
|---|---|
| Target | Angiopoietin 2 |
| Tissue Specificity | Recognizes human and mouse angiopoietin-2. Does not detect human angiopoietin-1. |
| Light Chain Type | λ |
Application
Structure of the recombinant antibody anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a mouse IgG2b Fc region.
Application
Binding of human Angiopoietin-2 to Tie-2 (human):Fc is inhibited by the antibody anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free). Methods: Tie-2 (h):Fc was coated on an ELISA plate at 1µg/ml. anti-Angiopoietin-2, mAb (rec.) (blocking) (Angy-2-1) (preservative free) or an unrelated mAb (recombinant) (Control) were added (starting at 40µg/ml with a twofold serial dilution) together with 20ng/µl of Angiopoietin-2 (human). After incubation for 1h at RT, the binding was detected using an anti-FLAG antibody (HRP).| Application Notes | Functional: Inhibits the binding of mouse angiopoietin-2 to mouse Tie-2. ND50 = 50-60ng/ml (for 10ng/ml of mouse Angiopoietin-2) inhibits the binding of human angiopoietin-2 to human Tie-2. ND50 = 8-12ng/ml (for 10ng/ml of human Angiopoietin-2) ND50: 50% neutralizing dose of antibody for a given concentration of ligand (here Angiopoietin-2). |
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| Form | Liquid |
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| Storage Instructions | Stable for at least 1 year after receipt when stored at -20°C. |
| Storage Buffer | PBS containing 10% glycerol. |
