Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Annexin A1 antibody at 1:500 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin A1 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:10000Application
Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000| Product Name | Annexin A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Annexin A1 (annexin A1)Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Cat, Pig, Rabbit, Chimpanzee, BovineRat (89%), Dog (92%), Cat (93%), Pig (87%), Rabbit (92%), Chimpanzee (99%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ANX1 antibody, LPC1 antibody, ANXA1 antibody, calpactin-2 antibody, chromobindin-9 antibody, annexin-1 antibody, phospholipase A2 inhibitory protein antibody, calpactin II antibody, lipocortin I antibody, annexin I (lipocortin I) antibody, annexin A1 antib |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) (2) |
| Cellular Localization | Nucleus , Cytoplasm , Cell projection , cilium , Basolateral cell membrane |
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Annexin A1 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin A1 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:10000
Application
Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000| Positive Control | H1299 , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
