Application
Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 μg of Annexin A1 antibody . Western blot analysis was performed using Annexin A1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin I antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded N87 Xenograft , using Annexin I antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 C: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Annexin A1 antibody detects Annexin A1 protein at cytosol and nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Annexin A1 antibody dilution: 1:500.| Product Name | Annexin A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Annexin A1 (annexin A1)Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Pig, Rabbit, Chimpanzee, BovineDog (91%), Pig (88%), Rabbit (91%), Chimpanzee (100%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ANX1 antibody, LPC1 antibody, ANXA1 antibody, calpactin-2 antibody, chromobindin-9 antibody, annexin-1 antibody, phospholipase A2 inhibitory protein antibody, calpactin II antibody, lipocortin I antibody, annexin I (lipocortin I) antibody, annexin A1 antib |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm , Cell projection , cilium , Basolateral cell membrane |
Application
Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 μg of Annexin A1 antibody . Western blot analysis was performed using Annexin A1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Annexin I antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded N87 Xenograft , using Annexin I antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 C: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Annexin A1 antibody detects Annexin A1 protein at cytosol and nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Annexin A1 antibody dilution: 1:500.| Positive Control | A431 , H1299 , HeLa , Molt-4 , NIH-3T3 , JC |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
