Application
Western blot analysis of Anthrax LF in recombinant protein using at 0.1 mg/ml.| Product Name | Anthrax LF antibody [5F502.2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [5F502.2] to Anthrax LFAnthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxicity to occur. It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63 kD carboxyl-terminal fragment binds to LF and EF. LF is a protease that is known to cleave members of the mitogen-activated protein kinase kinase (MAPKK) family. |
| Immunogen | Synthetic peptide, corresponding to amino acids 779-792 of Anthrax LF |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5F502.2 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: Use at a concentration of 0.1 μg/ml. Detects a band of approximately ~90 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | Anthrax lethal factor bacillus anthracis lethal factor |
|---|---|
| Target | Anthrax LF |
Application
Western blot analysis of Anthrax LF in recombinant protein using at 0.1 mg/ml.| Application Notes | WB: Use at a concentration of 0.1 μg/ml. Detects a band of approximately ~90 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 0.2% Gelatin, PBS |
