Application
Immunohistochemical staining of human heart with Rabbit anti APAF1Application
Western Blot analysis of whole cell lysate from K562 cells probed with Rabbit anti APAF1 either in the absence (A) or presence (B) of blocking peptide| Product Name | APAF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to APAF1Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1 (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 transcript is ubiquitously expressed in human tissues (1). |
| Immunogen | Synthetic peptide, corresponding to amino acids 12-28 of Human Apaf 1. The sequences of the immunogenic peptide are identical between human and mouse. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| For WB: Use at a dilution of 1:1,000 to 1:2,000. Optimal concentrations/dilutions should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CED4, APAF1, 602233, O14727, 317 |
|---|---|
| Target | APAF1 |
| Tissue Specificity | Full length Apaf1 |
| Cellular Localization | Cytoplasmic |
Application
Immunohistochemical staining of human heart with Rabbit anti APAF1
Application
Western Blot analysis of whole cell lysate from K562 cells probed with Rabbit anti APAF1 either in the absence (A) or presence (B) of blocking peptide| Positive Control | HeLa whole cell lysate. |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:1,000 to 1:2,000. Optimal concentrations/dilutions should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide |
