Application
Human Colon Carcinoma stained with anti-Adenomatous Polyposis Coli (APC) antibody| Product Name | APC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to APCThis gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| We recommend the following starting dilutions: For IHC-P: Use at a dilution of 1:100. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DP2.5, 324, FAP, DP3, GS, 135290, DP2, P25054, APC, FPC |
|---|---|
| Target | APC |
Application
Human Colon Carcinoma stained with anti-Adenomatous Polyposis Coli (APC) antibody| Positive Control | Colon carcinoma |
|---|---|
| Application Notes | We recommend the following starting dilutions: For IHC-P: Use at a dilution of 1:100. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions should be determined experimentally by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
