Mouse monoclonal [c-APC 28.9] to APCUseful for APC expression and mutation detection. It has been used routinely for Western blotting on colon cell line HCT116 extract that expresses full-length APC.
Immunogen
human APC C-terminal fusion with MBP (maltose binding protein)
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
c-APC 28.9
Host Species
Mouse
Tested Applications
ICC/IFIHC-FIPWB
IF: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 300 kDa. Optimal dilutions/concentrations should be determined by the end user.
Species Reactivity
HumanMouse
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
This antibody has been used routinely for Western blotting on colon cell line HCT116 extract which expresses full-length APC.
Light Chain Type
kappa
Positive Control
colon cell line HCT116
Application Notes
IF: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 300 kDa. Optimal dilutions/concentrations should be determined by the end user.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.