| Product Name | APC antibody [FE9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [FE9] to APCThis gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. |
| Immunogen | Immunogen used was a synthetic peptide corresponding to the N-terminal 35 amino acid residues of APC. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | FE9 |
| Host Species | Mouse |
| Tested Applications | WB |
| For WB: Use at a concentration of 1 - 1.5μg/ml. Predicted molecular weight: 312 kDa. (SW480 cells, which contain a mutation at codon 1338 of APC and express atruncated APC protein of 147 kDa). Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | DP2.5, 324, FAP, DP3, GS, 135290, DP2, P25054, APC, FPC, BTPS2 |
|---|---|
| Target | APC |
| Tissue Specificity | Reacts with all full-length and truncated APC proteins |
| Cellular Localization | Secreted |
| Positive Control | HCT116 cells for p300, SW480 cells for truncated APC (p147) |
|---|---|
| Application Notes | For WB: Use at a concentration of 1 - 1.5μg/ml. Predicted molecular weight: 312 kDa. (SW480 cells, which contain a mutation at codon 1338 of APC and express atruncated APC protein of 147 kDa). Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 50 mM sodium phosphate buffer, pH 7.5, 0.2% gelatin with =0.1% sodium azide |
