Application
Cross-linked ChIP was performed with 293T chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-APE1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MDR1 promoter.Application
APE1 antibody immunoprecipitates APE1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : 30 μg whole cell lysate/extract of APE1 protein expressing A431 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of APE1 by 3 μg of APE1 antibody 10% SDS-PAGE The immunoprecipitated APE1 protein was detected by APE1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Application
APE1 antibody detects APE1 protein at cytoplasm, nucleus and nucleolus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. APE1 antibody diluted at 1:500.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using APE1 antibody at 1:200 dilution.Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
APE1 antibody detects APEX1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10% SDS-PAGE APE1 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | APE1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to APE1 (APEX nuclease (multifunctional DNA repair enzyme) 1)Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human APE1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Pig, Rhesus Monkey, Chimpanzee, BovineDog (95%), Pig (94%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | APE antibody, APE1 antibody, APEN antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody, APEX1 antibody, apurinic-apyrimidinic endonuclease 1 antibody, deoxyribonuclease (apurinic or apyrimidinic) antibody, apurinic/apyrimidinic (abasic) endo |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Cross-linked ChIP was performed with 293T chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-APE1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MDR1 promoter.
Application
APE1 antibody immunoprecipitates APE1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : 30 μg whole cell lysate/extract of APE1 protein expressing A431 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of APE1 by 3 μg of APE1 antibody 10% SDS-PAGE The immunoprecipitated APE1 protein was detected by APE1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Application
APE1 antibody detects APE1 protein at cytoplasm, nucleus and nucleolus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. APE1 antibody diluted at 1:500.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using APE1 antibody at 1:200 dilution.
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
APE1 antibody detects APEX1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10% SDS-PAGE APE1 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , mouse brain , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
