Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:3000Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody [N1], N-term diluted at 1:2000.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using APE1 antibody at 1:200 dilution.Application
APE1 antibody [N1], N-term detects APEX1 protein at nucleus on Saos2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Saos2 xenograft. APE1 antibody [N1], N-term dilution: 1:500.| Product Name | APE1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to APE1 (APEX nuclease (multifunctional DNA repair enzyme) 1)Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human APE1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (100%), Chimpanzee (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | APE antibody, APE1 antibody, APEN antibody, APEX antibody, APX antibody, HAP1 antibody, REF1 antibody, APEX1 antibody, apurinic-apyrimidinic endonuclease 1 antibody, deoxyribonuclease (apurinic or apyrimidinic) antibody, apurinic/apyrimidinic (abasic) endo |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:3000
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody [N1], N-term diluted at 1:2000.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using APE1 antibody at 1:200 dilution.
Application
APE1 antibody [N1], N-term detects APEX1 protein at nucleus on Saos2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Saos2 xenograft. APE1 antibody [N1], N-term dilution: 1:500.| Positive Control | A549 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
