Application
Western Blot: Apolipoprotein A5 Antibody (1G5G9) [ - Detection of Apo A5 in HepG2 cell lysate withApplication
Western Blot: Apolipoprotein A5 Antibody (1G5G9) [ - Analysis using anti- human APOA5 monoclonal antibody against human serum total protein (lane 2). Lane 1 is a MW standard| Product Name | Apolipoprotein A5 antibody [1G5G9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monclonal antibody [1G5G9] to Apolipoprotein A5Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model with human ApoA5 produced 50n70% lower concentrations of mouse serum triglycerides. In addition, peroxisome proliferator-activated receptor-4 (PPAR-alpha) agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Recently, it was demonstrated that ApoA5 is present in human serum detected by polyclonal antibodies against both the NH2 and COOH termini, although at much lower concentration than other apolipoproteins. |
| Immunogen | Ni-NTA purified truncated recombinant human APOA5 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1G5G9 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| This antibody has been tested in ELISA and Western Blot. The MW of this protein is ~41 kDa. Suggested working dilutions: *Western Blot - 1:500-1:2,000 (ECL)Immunohistochemistry - ND*The investigator should determine the optimal working dilution for a specific application. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Apo A5, APOA-V, MGC126838, MGC126836, APOAV, Q6Q788, RAP3, 144650, APOA5, 116519, APOA V |
|---|---|
| Target | Apo A5 |
Application
Western Blot: Apolipoprotein A5 Antibody (1G5G9) [ - Detection of Apo A5 in HepG2 cell lysate with
Application
Western Blot: Apolipoprotein A5 Antibody (1G5G9) [ - Analysis using anti- human APOA5 monoclonal antibody against human serum total protein (lane 2). Lane 1 is a MW standard| Application Notes | This antibody has been tested in ELISA and Western Blot. The MW of this protein is ~41 kDa. Suggested working dilutions: *Western Blot - 1:500-1:2,000 (ECL)Immunohistochemistry - ND*The investigator should determine the optimal working dilution for a specific application. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.1 ml of crude ascites (3.6 mg/ml) containing 0.1% sodium azide. |
