Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)Application
Flow cytometric analysis of serun using ApoB mouse mAb (green) and negative control (purple)Application
Western blot analysis using ApoB mAb against human ApoB (AA: 3900-4110) recombinant protein. (Expected MW is 45 kDa)Application
Immunofluorescence analysis of HepG2 cells using ApoB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin| Product Name | Apolipoprotein B antibody [6G6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6G6] to Apolipoprotein BThis gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 3900 and 4110 of human ApoB. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6G6 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. Not yet tested in other applications. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FLDB , APOB , LDLCQ4 |
|---|---|
| Target | Apolipoprotein B |
| Molecular Weight(MW) | 516 kDa (note) |
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)
Application
Flow cytometric analysis of serun using ApoB mouse mAb (green) and negative control (purple)
Application
Western blot analysis using ApoB mAb against human ApoB (AA: 3900-4110) recombinant protein. (Expected MW is 45 kDa)
Application
Immunofluorescence analysis of HepG2 cells using ApoB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin| Application Notes | Recommended Starting Dilutions: ELISA: 1/10000. WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. Not yet tested in other applications. Determining optimal working dilutions by titration test. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
