| Product Name | Apolipoprotein E antibody [E6D7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [E6D7] to Apolipoprotein EApolipoprotein E has been shown to enhance the risk for the development of Alzheimeris disease. Mutations in the APP gene in conjunction with the influence of ApoE appears to influence amyloid metabolism. The ApoE antibody (D6E10) reacts with the E2, E3 and E4 isoforms of apolipoprotein E. ApoE4 can be identified by Western blot analysis using clones E6D7 and D6E10 (GTX21906). |
| Immunogen | Synthetic peptide spanning the polymorphic amino acid position 158 of ApoE. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | E6D7 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-FIHC-PIPWB |
| ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. Staining may be significantly improved by either1. Formic acid (70%) for 10-30 minutes at room temperature2. HIER (heat Induced Epitope Retrieval) with buffers such as Citrate IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/100 - 1/1000. Predicted molecular weight: 38 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | MGC1571, apoprotein, AD2, APOE, Apo E, P02649, 348, 104310, LDLCQ5, LPG |
|---|---|
| Target | Apolipoprotein E |
| Application Notes | ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. Staining may be significantly improved by either1. Formic acid (70%) for 10-30 minutes at room temperature2. HIER (heat Induced Epitope Retrieval) with buffers such as Citrate IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/100 - 1/1000. Predicted molecular weight: 38 kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4ºC for short-term. For long-term storage store at -20ºC. Avoid repeated freeze and thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS |
