Application
Sandwich ELISA analysis of human apolipoprotein E using Mouse anti Human apolipoprotein E as a capture reagent and biotinylated Goat anti Human Apolipoprotein E as a detection reagent. A serum sample 1/150 (green) and a plasma sample 1/150 (orange) are shown.Application
ELISA analysis of human apolipoprotein E protein using Apolipoprotein E antibody [WUE-4] (Low endotoxin, azide free).| Product Name | Apolipoprotein E antibody [WUE-4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [WUE-4] to Apolipoprotein EChylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jul 2008] |
| Immunogen | Purified ApoHDL fraction |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | WUE-4 |
| Host Species | Mouse |
| Tested Applications | ELISAWBFunctional Assay |
| ELISA: 1/100 - 1/1000 Optimal dilution / concentration should be determined by the end user. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | apolipoprotein E , AD2 , APOE , LPG , LDLCQ5 |
|---|---|
| Target | Apolipoprotein E |
| Tissue Specificity | This antibody recognizes an epitope within amino acids 140-160 of human Apolipoprotein E. |
Application
Sandwich ELISA analysis of human apolipoprotein E using Mouse anti Human apolipoprotein E as a capture reagent and biotinylated Goat anti Human Apolipoprotein E as a detection reagent. A serum sample 1/150 (green) and a plasma sample 1/150 (orange) are shown.
Application
ELISA analysis of human apolipoprotein E protein using Apolipoprotein E antibody [WUE-4] (Low endotoxin, azide free).| Application Notes | ELISA: 1/100 - 1/1000 Optimal dilution / concentration should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline |
