Application
IHC analysis of temporal lobe section from a patient with Alzheimer’s disease using APP antibody [DE4B2]. Plaques in the neuropil have been circled in black.| Product Name | APP antibody [DE2B4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [DE2B4] to APPAmyloid Precursor Protein gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. |
| Immunogen | Synthetic peptide corresponding to beta Amyloid peptide (1-17), conjugated to KLH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | DE2B4 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| ICC: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature before commencing with IHC staining protocol. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/50 - 1/500. Predicted molecular weight: kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMonkeyPigBovine |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Target | APP |
|---|---|
| Tissue Specificity | Reacts with beta-amyloid peptide and secreted amyloid precursor protein (alpha-secretase cleaved). |
| Cellular Localization | Endoplasmic reticulum, Golgi Apparatus, Cytoplasmic, Cell Membrane. |
Application
IHC analysis of temporal lobe section from a patient with Alzheimer’s disease using APP antibody [DE4B2]. Plaques in the neuropil have been circled in black.| Application Notes | ICC: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. Retrieve antigens with 70% Formic acid for 10-30 minutes at room temperature before commencing with IHC staining protocol. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/50 - 1/500. Predicted molecular weight: kDa. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C. |
| Storage Buffer | Preservative: 0.01% Sodium Azide |
