Application
Co-localization of ARAP1 with DR4 at the plasma membrane and in early endosomes. ARAP1-transfected NCTC cells were treated with TRAIL for 0´, 30´ or 60´ and fixed with methanol. DR4 was detected by rabbit polyclonal (red) and ARAP1 with ARAP1-2 mouse monoclonal antibody (green). Arrowheads show ARAP1-DR4 co-localization at the plasma membrane and intracellular membranes, whereas arrows show their co-localization in early endosomes.| Product Name | ARAP1 antibody [ARAP1-2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [ARAP1-2] to ARAP1ARAP1 (angiotensin II type 1 receptor-associated protein) is an adaptor protein with ArfGAP and RhoGAP activities, containing five PH domains and ankyrin repeate. This adaptor seems to serve as a link between phosphoinositide- Arf-, and Rho-mediated cell signaling. ARAP1 supports the plasma membrane recycling of angiotensin II type 1 receptor (AT1) and is important also for cell-specific trafficking of pro-death receptor TRAIL-R1 (DR4) to the plasma membrane, thus promoting the TRAIL-induced apoptosis in certain cell types. ARAP1 also affects cell spreading.Entrez Gene: 11q13.4 |
| Immunogen | His6-tagged protein corresponding to amino acids 1190-1450 of human ARAP1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | ARAP1-2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| WB: Use at 2 µg/mL | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ARAP1, KIAA0782, centaurin |
|---|---|
| Target | ARAP1 |
| Tissue Specificity | The antibody ARAP1-2 reacts with C-terminal part of ARAP1, a 160 kDa adaptor protein. |
| Cellular Localization | Associated with Golgi stacks in resting cells. Throughout the cytoplasm and in surface protrusion in cells that are in the process of attaching to a surface and spreading. |
Application
Co-localization of ARAP1 with DR4 at the plasma membrane and in early endosomes. ARAP1-transfected NCTC cells were treated with TRAIL for 0´, 30´ or 60´ and fixed with methanol. DR4 was detected by rabbit polyclonal (red) and ARAP1 with ARAP1-2 mouse monoclonal antibody (green). Arrowheads show ARAP1-DR4 co-localization at the plasma membrane and intracellular membranes, whereas arrows show their co-localization in early endosomes.| Application Notes | WB: Use at 2 µg/mL |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
