Application
Western blot analysis of ARC in HeLa cell lysate with ARC antibody at 2ug/ml dilution.| Product Name | ARC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ARCApoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICKBCL10, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD (1). ARC interacts withcaspase-2 and ~8 and inhibits enzymatic activity ofcaspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1, and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue (1). |
| Immunogen | Synthetic peptide - amino acids 2-18 of ARC |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:500. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NOP30, ARC, NOP, 8996, O60936, 605235, NOL3, MYC |
|---|---|
| Target | ARC |
Application
Western blot analysis of ARC in HeLa cell lysate with ARC antibody at 2ug/ml dilution.| Positive Control | HeLa whole cell lysate |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500. Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide |
