Application
Western blot of ADP Ribosylation Factor in canine heart extract.Application
Flow cytometry analysis of ADP Ribosylation Factor in HeLa cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ADP Ribosylation Factor antibody [1D9] at a dilution of 1:80 for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a proper secondary antibody, and re-suspended in PBS for FACS analysis.Application
Immunofluorescent analysis of ADP Ribosylation Factor in HeLa Cells. ADP Ribosylation Factor staining (green) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ADP Ribosylation Factor antibody [1D9] at a dilution of 1:100 over night at 4 °C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.Application
Immunohistochemistry was performed on cancer biopsies of deparaffinized human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with or without ADP Ribosylation Factor antibody [1D9] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Product Name | ARF1/ARF3/ARF5/ARF6 antibody [1D9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1D9] to ARF1/ARF3/ARF5/ARF6 |
| Immunogen | Recombinant human ARF1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1D9 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PIPWB |
| ICC/IF: 1:500 | |
| Species Reactivity | HumanMouseDogRatHamsterPrimateRabbitBovine |
| Concentration | 1mg/ml |
| Target | ADP-Ribosylation Factor |
|---|---|
| Tissue Specificity | This antibody detects Arf1, Arf3, Arf5 and Arf6 and about ten-fold less well with Arf4 from human, mouse and rat tissues. |
Application
Western blot of ADP Ribosylation Factor in canine heart extract.
Application
Flow cytometry analysis of ADP Ribosylation Factor in HeLa cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with ADP Ribosylation Factor antibody [1D9] at a dilution of 1:80 for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a proper secondary antibody, and re-suspended in PBS for FACS analysis.
Application
Immunofluorescent analysis of ADP Ribosylation Factor in HeLa Cells. ADP Ribosylation Factor staining (green) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ADP Ribosylation Factor antibody [1D9] at a dilution of 1:100 over night at 4 °C, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.
Application
Immunohistochemistry was performed on cancer biopsies of deparaffinized human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with or without ADP Ribosylation Factor antibody [1D9] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Application Notes | ICC/IF: 1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | Ascites containing 0.05 % sodium azide |
