Application
Immunofluorescence analysis of methanol-fixed HeLa, using ARF3 antibody at 1:500 dilution.Application
ARF3 antibody detects ARF3 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. ARF3 antibody dilution: 1:500.Application
ARF3 antibody detects ARF3 protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. ARF3 antibody dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE ADP-ribosylation factor 3 antibody diluted at 1:1000| Product Name | ARF3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ARF3 (ADP-ribosylation factor 3)ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ARF3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, Sheep, BovineRat (100%), Xenopus laevis (100%), Pig (100%), Sheep (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARF3 antibody, small GTP binding protein antibody, ADP-ribosylation factor 3 antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) |
| Cellular Localization | Golgi apparatus |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ARF3 antibody at 1:500 dilution.
Application
ARF3 antibody detects ARF3 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. ARF3 antibody dilution: 1:500.
Application
ARF3 antibody detects ARF3 protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. ARF3 antibody dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE ADP-ribosylation factor 3 antibody diluted at 1:1000| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
