Rabbit polyclonal antibody to ADP-ribosylation factor 3 (ADP-ribosylation factor 3)ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human ARF3. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
Mouse
Predicted Reactivity
Human, Rat, Xenopus laevis, Pig, Sheep, BovineHuman (100%), Rat (100%), Xenopus laevis (99%), Pig (99%), Sheep (100%), Bovine (100%)
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
ARF3 antibody, small GTP binding protein antibody, ADP-ribosylation factor 3 antibody
Molecular Weight(MW)
21 kDa (note) (2)
Cellular Localization
Golgi apparatus
Application
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000
Positive Control
Mouse brain
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.