Application
IP analysis of extracts from HeLa subjected to UV crosslinking using Argonautes antibody and control mouse IgG. The immunoprecipitants were detected by Argonautes antibody. 10% of input was also loaded for analysis.Application
Co-IP of RNA-Argonaute complex using Argonautes antibody. HeLa cells were transfected with miR-1 target allele, miR-1C target allel and miRNA (miR-1, miR-1C or miR-377). Cell lysate was immunoprecipitated with ith Argonautes antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. The predicted miRNA target alleles were efficiently co-immunoprecipitated by Argonautes antibody.| Product Name | Argonautes antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to Argonautes |
| Clonality | Monoclonal |
|---|---|
| Clone Number | GT9003 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPRIPWBChIP |
| IP (RIP)( 8 μg), ChIP-seq, IHC(1:500), IF(1:500), WB(1:300-1:500) | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Ago (Argonautes) |
|---|
Application
IP analysis of extracts from HeLa subjected to UV crosslinking using Argonautes antibody and control mouse IgG. The immunoprecipitants were detected by Argonautes antibody. 10% of input was also loaded for analysis.
Application
Co-IP of RNA-Argonaute complex using Argonautes antibody. HeLa cells were transfected with miR-1 target allele, miR-1C target allel and miRNA (miR-1, miR-1C or miR-377). Cell lysate was immunoprecipitated with ith Argonautes antibody. RNA was isolated from the complexes from the input and IP samples, reverse transcribed using random hexamers, and subjected to RT-PCR to confirm enrichment of miRNA targeted allele in IP sample compared to input sample. The PCR products were directly sequenced. The electropherograms at the polymorphic site are shown in the second panel. The predicted miRNA target alleles were efficiently co-immunoprecipitated by Argonautes antibody.| Application Notes | IP (RIP)( 8 μg), ChIP-seq, IHC(1:500), IF(1:500), WB(1:300-1:500) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide; affinity purified using Protein A. |
