Application
HEK293T cells transfected with either RC200902 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARHGDIA antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGDIA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGDIA.Application
Flow cytometric Analysis of Jurkat cells, using anti-ARHGDIA antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Flow cytometric Analysis of Hela cells, using anti-ARHGDIA antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGDIA monoclonal antibody.| Product Name | ARHGDIA antibody [1F2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1F2] to ARHGDIARegulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. |
| Immunogen | Full length human recombinant protein of human ARHGDIA (NP_004300) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1F2 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| IHC 1:50 | |
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC117248, GDIA1, RHOGDI, ARHGDIA |
|---|---|
| Target | ARHGDIA |
| Tissue Specificity | is specific for human ARHGDIA (NP_004300). |
Application
HEK293T cells transfected with either RC200902 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARHGDIA antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGDIA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGDIA.
Application
Flow cytometric Analysis of Jurkat cells, using anti-ARHGDIA antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Flow cytometric Analysis of Hela cells, using anti-ARHGDIA antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGDIA monoclonal antibody.| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
