Application
THP-1 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [GT18112] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.Application
Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [GT18112] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced. (LPS: Lipopolysaccharides)Application
ARID5A antibody detects ARID5A protein by western blot analysis. Non-transfected (-) and ARID5A-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody diluted by 1:5000.| Product Name | ARID5A antibody [GT18112] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT18112] to ARID5A (AT rich interactive domain 5A (MRF1-like))Members of the ARID protein family, including ARID5A, have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth (Patsialou et al., 2005 [PubMed 15640446]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ARID5A. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT18112 |
| Host Species | Mouse |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | BovineBovine (88%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AT rich interactive domain 5A (MRF1-like) , MRF-1 , RP11-363D14 , MRF1 |
|---|---|
| Target | ARID5A |
| Molecular Weight(MW) | 64 kDa (note) |
| Cellular Localization | Nucleus |
Application
THP-1 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [GT18112] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
Application
Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody [GT18112] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced. (LPS: Lipopolysaccharides)
Application
ARID5A antibody detects ARID5A protein by western blot analysis. Non-transfected (-) and ARID5A-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARID5A antibody diluted by 1:5000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
