Application
Anti-ARNTL mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with ARNTLApplication
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARNTL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNTL.Application
HEK293T cells transfected with either RC207870 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARNTL antibody, and then analyzed by flow cytometry.Application
Flow cytometric Analysis of Jurkat cells, using anti-ARNTL antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARNTL monoclonal antibody.| Product Name | ARNTL antibody [3G9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3G9] to ARNTLARNTL-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. This transcription is inhibited in a feedback loop by PER, and also by CRY proteins. |
| Immunogen | Full length human recombinant protein of human ARNTL (NP_001169) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3G9 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 | |
| Species Reactivity | HumanDogRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MOP3, BMAL1c, TIC, MGC47515, JAP3, ARNTL, BMAL1, PASD3 |
|---|---|
| Target | ARNTL |
| Tissue Specificity | is specific for human ARNTL (NP_001169). |
Application
Anti-ARNTL mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with ARNTL
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARNTL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNTL.
Application
HEK293T cells transfected with either RC207870 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARNTL antibody, and then analyzed by flow cytometry.
Application
Flow cytometric Analysis of Jurkat cells, using anti-ARNTL antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARNTL monoclonal antibody.| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
