Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody diluted at 1:1000.Application
ASCL1 antibody detects ASCL1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15 % SDS-PAGE, and blotted with ASCL1 antibody diluted by 1:1000Application
SH-SY5Y whole cell and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody diluted at 1:1000.Application
ASCL1 antibody detects ASCL1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ASCL1 antibody diluted at 1:500.Application
Various whole cell and SH-SY5Y nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody diluted at 1:1000.| Product Name | ASCL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ASCL1 (achaete-scute complex homolog 1 (Drosophila))This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ASCL1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | MouseMouse (84%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | bHLHa46 MASH1 HASH1 achaete-scute complex homolog 1 (Drosophila) ASH1 |
|---|---|
| Target | ASCL1 |
| Molecular Weight(MW) | 25 kDa (note) |
| Cellular Localization | Nucleus |
Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody diluted at 1:1000.
Application
ASCL1 antibody detects ASCL1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15 % SDS-PAGE, and blotted with ASCL1 antibody diluted by 1:1000
Application
SH-SY5Y whole cell and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody diluted at 1:1000.
Application
ASCL1 antibody detects ASCL1 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ASCL1 antibody diluted at 1:500.
Application
Various whole cell and SH-SY5Y nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody diluted at 1:1000.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS , SH-SY5Y , SH-SY5Y nuclear extract , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
