Application
IHC analysis of human breast carcinoma tissue using ATF1 (phospho Ser63) antibody [EP1591(2)Y] at a a dilution of 1:100.Application
WB analysis of (1) untreated and (2) Lambda Phosphatase-treated HeLa lysates (10 μg per lane) using ATF1 (phospho Ser63) antibody [EP1591(2)Y] at a dilution of 1:100,000.| Product Name | ATF1 (phospho Ser63) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP1591(2)Y] to ATF1 (phospho Ser63)This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010] |
| Immunogen | A phospho specific peptide corresponding to residues surrounding serine 63 of human ATF1 |
| Modification | p-Ser63 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP1591(2)Y |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | ATF1 Phospho ATF1 S63 Phospho ATF1 (Phospho Ser63) Phospho ATF1 S63 ATF1 (Phospho S63) ATF1 Phospho S63 FUS/ATF-1 TREB36 EWS-ATF1 ATF1 (pS63) Pho |
|---|---|
| Target | ATF1 (phospho Ser63) |
| Tissue Specificity | This antibody detects ATF1 when phosphorylated at serine 63 |
Application
IHC analysis of human breast carcinoma tissue using ATF1 (phospho Ser63) antibody [EP1591(2)Y] at a a dilution of 1:100.
Application
WB analysis of (1) untreated and (2) Lambda Phosphatase-treated HeLa lysates (10 μg per lane) using ATF1 (phospho Ser63) antibody [EP1591(2)Y] at a dilution of 1:100,000.| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% Glycerol, 0.05% BSA and 0.01% Sodium azide. |
